4ZM9

Crystal structure of circularly permuted human asparaginase-like protein 1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004067	molecular_function	asparaginase activity
A	0016787	molecular_function	hydrolase activity
B	0004067	molecular_function	asparaginase activity
B	0016787	molecular_function	hydrolase activity
C	0004067	molecular_function	asparaginase activity
C	0016787	molecular_function	hydrolase activity
D	0004067	molecular_function	asparaginase activity
D	0016787	molecular_function	hydrolase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue NA A 401

Chain	Residue
A	LEU55
A	GLU56
A	ASP58
A	PHE61
A	ALA63
A	CYS65

---

site_id	AC2
Number of Residues	11
Details	binding site for residue GLY A 402

Chain	Residue
A	ARG196
A	ASP199
A	SER200
A	GLY220
A	GLY222
A	DTT407
A	NA408
A	HOH510
A	THR168
A	GLY188
A	ILE189

---

site_id	AC3
Number of Residues	3
Details	binding site for residue BME A 403

Chain	Residue
A	GLU239
B	LEU233
B	PHE236

---

site_id	AC4
Number of Residues	8
Details	binding site for residue BME A 404

Chain	Residue
A	SER88
A	ALA89
A	VAL108
A	MET109
A	THR112
A	HIS114
A	CYS115
B	LYS227

---

site_id	AC5
Number of Residues	5
Details	binding site for residue BME A 405

Chain	Residue
A	BME406
A	HOH519
B	GLY36
B	ARG39
B	PRO308

---

site_id	AC6
Number of Residues	6
Details	binding site for residue BME A 406

Chain	Residue
A	GLY26
A	ARG29
A	ALA54
A	ASP58
A	BME405
B	PRO308

---

site_id	AC7
Number of Residues	6
Details	binding site for residue DTT A 407

Chain	Residue
A	GLY11
A	THR168
A	VAL190
A	THR219
A	GLY220
A	GLY402

---

site_id	AC8
Number of Residues	5
Details	binding site for residue NA A 408

Chain	Residue
A	THR168
A	ASP199
A	SER200
A	THR218
A	GLY402

---

site_id	AC9
Number of Residues	6
Details	binding site for residue NA B 401

Chain	Residue
B	LEU55
B	GLU56
B	ASP58
B	PHE61
B	ALA63
B	CYS65

---

site_id	AD1
Number of Residues	10
Details	binding site for residue GLY B 402

Chain	Residue
B	THR168
B	GLY188
B	ARG196
B	ASP199
B	SER200
B	GLY220
B	GLY222
B	FLC403
B	HOH505
B	HOH507

---

site_id	AD2
Number of Residues	8
Details	binding site for residue FLC B 403

Chain	Residue
B	GLY11
B	ASN62
B	ARG147
B	THR168
B	ILE189
B	THR219
B	GLY220
B	GLY402

---

site_id	AD3
Number of Residues	8
Details	binding site for residue BME B 404

Chain	Residue
A	LYS227
B	SER88
B	ALA89
B	VAL108
B	MET109
B	THR112
B	HIS114
B	CYS115

---

site_id	AD4
Number of Residues	6
Details	binding site for residue NA C 401

Chain	Residue
C	LEU55
C	GLU56
C	ASP58
C	PHE61
C	ALA63
C	CYS65

---

site_id	AD5
Number of Residues	10
Details	binding site for residue GLY C 402

Chain	Residue
C	THR168
C	GLY188
C	ARG196
C	ASP199
C	SER200
C	GLY220
C	GLY222
C	ILE225
C	NA409
C	HOH506

---

site_id	AD6
Number of Residues	8
Details	binding site for residue BME C 403

Chain	Residue
C	ALA89
C	VAL108
C	MET109
C	THR112
C	HIS114
C	CYS115
D	LYS227
C	SER88

---

site_id	AD7
Number of Residues	4
Details	binding site for residue BME C 404

Chain	Residue
C	GLY36
C	HOH509
D	ALA54
D	ASP57

---

site_id	AD8
Number of Residues	7
Details	binding site for residue BME C 405

Chain	Residue
C	VAL33
C	GLY50
C	ALA54
C	HOH513
D	THR32
D	GLY36
D	BME404

---

site_id	AD9
Number of Residues	7
Details	binding site for residue BME C 406

Chain	Residue
C	GLY26
C	ARG29
C	ALA54
C	LEU55
C	ASP58
D	PRO308
D	BME404

---

site_id	AE1
Number of Residues	5
Details	binding site for residue DTT C 407

Chain	Residue
C	GLY11
C	ASN62
C	THR168
C	THR219
C	GLY220

---

site_id	AE2
Number of Residues	4
Details	binding site for residue NA C 408

Chain	Residue
C	ASP78
C	ALA94
C	LYS192
C	ARG196

---

site_id	AE3
Number of Residues	5
Details	binding site for residue NA C 409

Chain	Residue
C	THR168
C	ASP199
C	SER200
C	THR218
C	GLY402

---

site_id	AE4
Number of Residues	6
Details	binding site for residue NA D 401

Chain	Residue
D	LEU55
D	GLU56
D	ASP58
D	PHE61
D	ALA63
D	CYS65

---

site_id	AE5
Number of Residues	11
Details	binding site for residue GLY D 402

Chain	Residue
D	THR168
D	GLY188
D	ILE189
D	ARG196
D	ASP199
D	SER200
D	GLY220
D	GLY222
D	FLC403
D	HOH509
D	HOH511

---

site_id	AE6
Number of Residues	8
Details	binding site for residue FLC D 403

Chain	Residue
D	GLY11
D	ASN62
D	THR168
D	GLY188
D	ILE189
D	THR219
D	GLY220
D	GLY402

---

site_id	AE7
Number of Residues	6
Details	binding site for residue BME D 404

Chain	Residue
C	VAL53
C	ALA54
C	ASP57
C	BME405
C	BME406
D	PRO308

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Nucleophile => ECO:0000269|PubMed:19839645, ECO:0000269|PubMed:22861376

Chain	Residue	Details
A	THR168
B	THR168
C	THR168
D	THR168

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	BINDING:

Chain	Residue	Details
A	ARG196
A	THR219
B	ARG196
B	THR219
C	ARG196
C	THR219
D	ARG196
D	THR219

---