4YYE

Crystal structure of the yeast mitochondrial threonyl-tRNA synthetase (MST1) in complex with the canonical tRNAThr and threonyl sulfamoyl adenylate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Threonine--tRNA ligase, mitochondrial	polymer	460	53473.9	2	UniProt (P07236) Pfam (PF00587) Pfam (PF03129)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Threonyl-tRNA synthetase,ThrRS
2	C, D (C, D)	tRNA	polymer	76	24328.3	2		Saccharomyces cerevisiae
3	E, J (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	F, K (A, B)	5'-O-(N-(L-THREONYL)-SULFAMOYL)ADENOSINE	non-polymer		447.4	2	Chemie (TSB)
5	G, H, I, L (A, B)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
6	M, N, O, P (A, B, C, D)	water	water		18.0	636	Chemie (HOH)

Sequence modifications

A, B: 26 - 462 (UniProt: P07236)

PDB	External Database	Details
Met 3	-	expression tag
Gly 4	-	expression tag
Ser 5	-	expression tag
Ser 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
Ser 13	-	expression tag
Ser 14	-	expression tag
Gly 15	-	expression tag
Leu 16	-	expression tag
Val 17	-	expression tag
Pro 18	-	expression tag
Arg 19	-	expression tag
Gly 20	-	expression tag
Ser 21	-	expression tag
His 22	-	expression tag
Met 23	-	expression tag
Ala 24	-	expression tag
Ser 25	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	155604.5
Non-Polymers*	Number of molecules	8
	Total formula weight	1409.9
All*	Total formula weight	157014.4

*Water molecules are not included.