4YYE
Crystal structure of the yeast mitochondrial threonyl-tRNA synthetase (MST1) in complex with the canonical tRNAThr and threonyl sulfamoyl adenylate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2014-08-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.03318 |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.021, 77.228, 86.157 |
| Unit cell angles | 63.86, 75.04, 89.39 |
Refinement procedure
| Resolution | 44.452 - 2.301 |
| R-factor | 0.1622 |
| Rwork | 0.160 |
| R-free | 0.20230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uh0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.122 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
| Rmerge | 0.103 | 0.063 | 0.312 |
| Total number of observations | 136098 | ||
| Number of reflections | 60476 | ||
| <I/σ(I)> | 6.3 | ||
| Completeness [%] | 88.9 | 86.2 | 90 |
| Redundancy | 2.3 | 2.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 285 | 0.2M lithium sulfate 2.0M ammonium sulfate 0.1M Tris |
