4Y5X

Diabody 305 complex with EpoR

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G, J	diabody 310 VL domain	polymer	136	14222.7	4		Homo sapiens
2	B, E, H, K	diabody 310 VH domain	polymer	116	11912.9	4	UniProt (Q5NV67) Pfam (PF07686) In PDB	Homo sapiens
3	C, F, I, L	Erythropoietin receptor	polymer	229	25129.4	4	UniProt (P19235) Pfam (PF09067) Pfam (PF00041) In PDB	Homo sapiens (Human)	EPO-R
4	L, A, B, C, E...	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	17	Chemie (PEG)
5	K	CITRATE ANION	non-polymer		189.1	1	Chemie (FLC)
6		water	water		18.0	28	Chemie (HOH)

Sequence modifications

B, E, H, K: 1 - 97 (UniProt: Q5NV67)

PDB	External Database	Details
Gly 15	Arg 15	conflict
Ala 21	Ser 21	conflict
Ser 35	Asn 35	conflict
Thr 46	Lys 46	conflict
Asn 53	Asp 53	conflict
Asp 98	-	expression tag
Trp 99	-	expression tag
Val 100	-	expression tag
Phe 101	-	expression tag
Gly 102	-	expression tag
Gly 103	-	expression tag
Gly 104	-	expression tag
Thr 105	-	expression tag
Lys 106	-	expression tag
Val 107	-	expression tag
Thr 108	-	expression tag
Val 109	-	expression tag
Leu 110	-	expression tag
Ala 111	-	expression tag
Ala 112	-	expression tag
Ala 113	-	expression tag
Gly 114	-	expression tag
Gly 115	-	expression tag
Gly 116	-	expression tag

C, F, I, L: 8 - 225 (UniProt: P19235)

PDB	External Database	Details
Phe 3	-	expression tag
Ala 4	-	expression tag
Gly 5	-	expression tag
Ser 6	-	expression tag
Ala 7	-	expression tag
Gln 52	Asn 76	engineered mutation
Gln 164	Asn 188	engineered mutation
Lys 226	-	expression tag
Glu 227	-	expression tag
Lys 228	-	expression tag
Ala 229	-	expression tag
Ala 230	-	expression tag
Ala 231	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	205060.1
Non-Polymers*	Number of molecules	18
	Total formula weight	1993.1
All*	Total formula weight	207053.3

*Water molecules are not included.