4Y1T

Structural basis for Ca2+-mediated interaction of the perforin C2 domain with lipid membranes

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Perforin-1	polymer	149	16475.1	1	UniProt (P10820) Pfam (PF00168) In PDB	Mus musculus (Mouse)	P1,Cytolysin,Lymphocyte pore-forming protein
2	A	CALCIUM ION	non-polymer		40.1	5	Chemie (CA)
3		water	water		18.0	63	Chemie (HOH)

Sequence modifications

A: 410 - 535 (UniProt: P10820)

PDB	External Database	Details
Ala 427	Trp 427	engineered mutation
Ala 430	Tyr 430	engineered mutation
Ala 486	Tyr 486	engineered mutation
Ala 488	Trp 488	engineered mutation
Gly 536	-	expression tag
Gln 537	-	expression tag
Ala 538	-	expression tag
Gly 539	-	expression tag
Gln 540	-	expression tag
His 541	-	expression tag
His 542	-	expression tag
His 543	-	expression tag
His 544	-	expression tag
His 545	-	expression tag
His 546	-	expression tag
Gly 547	-	expression tag
Ala 548	-	expression tag
Tyr 549	-	expression tag
Pro 550	-	expression tag
Tyr 551	-	expression tag
Asp 552	-	expression tag
Val 553	-	expression tag
Pro 554	-	expression tag
Asp 555	-	expression tag
Tyr 556	-	expression tag
Ala 557	-	expression tag
Ser 558	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	16475.1
Non-Polymers*	Number of molecules	5
	Total formula weight	200.4
All*	Total formula weight	16675.5

*Water molecules are not included.