4XR8
Crystal structure of the HPV16 E6/E6AP/p53 ternary complex at 2.25 A resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Maltose-binding periplasmic protein, ubiquitin ligase E6AP | polymer | 383 | 41803.3 | 2 | UniProt (P0AEX9) Pfam (PF01547) UniProt (by SIFTS) (Q05086) | Escherichia coli | MBP,MMBP,Maltodextrin-binding protein,MBP,MMBP,Maltodextrin-binding protein |
| 2 | C, D (C, D) | Cellular tumor antigen p53 | polymer | 199 | 22448.5 | 2 | UniProt (P04637) Pfam (PF00870) | Homo sapiens (Human) | Antigen NY-CO-13,Phosphoprotein p53,Tumor suppressor p53 |
| 3 | E, F (F, H) | Protein E6 | polymer | 151 | 18301.1 | 2 | UniProt (P03126) Pfam (PF00518) | Human papillomavirus type 16 | |
| 4 | G, H (E, G) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 5 | I, J, M, Q (A, B, D, F) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 4 | Chemie (PEG) | |||
| 6 | K, L, N, O, R... (C, D, F, H) | ZINC ION | non-polymer | 65.4 | 6 | Chemie (ZN) | |||
| 7 | P (F) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 8 | T, U, V, W, X... (A, B, C, D, F...) | water | water | 18.0 | 354 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 368 (UniProt: P0AEX9)
A, B: 372 - 383 (PDB: 4XR8)
F, H: 1 - 151 (UniProt: P03126)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Ala 83 | Asp 108 | engineered mutation |
| Ala 84 | Lys 109 | engineered mutation |
| Ala 240 | Lys 265 | engineered mutation |
| Ala 360 | Glu 385 | engineered mutation |
| Ala 363 | Lys 388 | engineered mutation |
| Ala 364 | Asp 389 | engineered mutation |
| Asn 368 | Arg 393 | linker |
| Ala 369 | - | linker |
| Ala 370 | - | linker |
| Ala 371 | - | linker |
F, H: 1 - 151 (UniProt: P03126)
| PDB | External Database | Details |
|---|---|---|
| Ser 80 | Cys 87 | engineered mutation |
| Ser 97 | Cys 104 | engineered mutation |
| Ser 111 | Cys 118 | engineered mutation |
| Ser 140 | Cys 147 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 165105.8 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 684.6 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 879.0 | |
| All* | Total formula weight | 166669.4 |
