4XGY

GFP based antibody (fluorobody)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Green fluorescent protein, mAb LCDR3, Green fluorescent protein	polymer	305	33817.8	1	UniProt (P42212) Pfam (PF01353) UniProt (by SIFTS) (A0A059PIQ0)	Aequorea victoria (Jellyfish)
2	B, C (A)	3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL	non-polymer		398.5	2	Chemie (PE5)
3	D (A)	water	water		18.0	303	Chemie (HOH)

Sequence modifications

A: 2 - 173 (UniProt: P42212)

PDB	External Database	Details
Met -6	-	expression tag
Lys -5	-	expression tag
Ala -4	-	expression tag
Thr -3	-	expression tag
Lys -2	-	expression tag
Leu -1	-	expression tag
Val 0	-	expression tag
Pro 1	-	expression tag
Arg 30	Ser 30	engineered mutation
Asn 39	Tyr 39	engineered mutation
Leu 64	Phe 64	engineered mutation
Cro 66	Ser 65	chromophore
Cro 66	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Arg 80	Gln 80	engineered mutation
Ser 99	Phe 99	engineered mutation
Thr 105	Asn 105	engineered mutation
Phe 145	Tyr 145	engineered mutation
Thr 153	Met 153	engineered mutation
Ala 163	Val 163	engineered mutation

A: 175 - 183 (PDB: 4XGY)
A: 187 - 251 (UniProt: P42212)

PDB	External Database	Details
Gly 174	-	linker
Thr 184	-	linker
Leu 185	-	linker
Gln 186	-	linker
Val 219	Ala 206	engineered mutation

A: 252 - 298 (PDB: 4XGY)

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	33817.8
Non-Polymers*	Number of molecules	2
	Total formula weight	797.0
All*	Total formula weight	34614.8

*Water molecules are not included.