4XGY

GFP based antibody (fluorobody)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97915
Spacegroup name	P 43 21 2
Unit cell lengths	63.350, 63.350, 125.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.675 - 1.494
R-factor	0.1461
Rwork	0.145
R-free	0.16650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.009
RMSD bond angle	1.335
Data reduction software	iMOSFLM
Data scaling software	iMOSFLM
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	31.680
High resolution limit [Å]	1.494
Number of reflections	42096
<I/σ(I)>	264.06
Completeness [%]	99.3
Redundancy	23.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	40% PEG400, 0.1M Hepes, pH 7.0