4XGY
GFP based antibody (fluorobody)
Summary for 4XGY
| Entry DOI | 10.2210/pdb4xgy/pdb |
| Related | 2b3p |
| Descriptor | Green fluorescent protein, mAb LCDR3, Green fluorescent protein, 3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL (3 entities in total) |
| Functional Keywords | fluorobody, gfp, cdr3, fluorescent protein |
| Biological source | Aequorea victoria (Jellyfish) More |
| Total number of polymer chains | 1 |
| Total formula weight | 34614.81 |
| Authors | Shi, N.,Chen, Y.G.,Wang, S.H. (deposition date: 2015-01-03, release date: 2015-07-01, Last modification date: 2023-11-15) |
| Primary citation | Chen, Y.,Huang, X.,Wang, R.,Wang, S.,Shi, N. The structure of a GFP-based antibody (fluorobody) to TLH, a toxin from Vibrio parahaemolyticus. Acta Crystallogr.,Sect.F, 71:913-918, 2015 Cited by PubMed Abstract: A fluorobody is a manmade hybrid molecule that is composed of green fluorescent protein (GFP) and a fragment of antibody, which combines the affinity and specificity of an antibody with the visibility of a GFP. It is able to provide a real-time indication of binding while avoiding the use of tags and secondary binding reagents. Here, the expression, purification and crystal structure of a recombinant fluorobody for TLH (thermolabile haemolysin), a toxin from the lethal food-borne disease bacterium Vibrio parahaemolyticus, are presented. This is the first structure of a fluorobody to be reported. Crystals belonging to space group P4(3)2(1)2, with unit-cell parameters a = b = 63.35, c = 125.90 Å, were obtained by vapour diffusion in hanging drops and the structure was refined to an Rfree of 16.7% at 1.5 Å resolution. The structure shows a CDR loop of the antibody on the GFP scaffold. PubMed: 26144238DOI: 10.1107/S2053230X15008845 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.494 Å) |
Structure validation
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