4X4V

Crystal structure of the A.fulgidus CCA-adding enzyme in complex with a human MenBeta minihelix ending in CCACC and AMPcPP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	CCA-adding enzyme	polymer	457	53672.5	2	UniProt (O28126) Pfam (PF01909) Pfam (PF09249) Pfam (PF21133)	Archaeoglobus fulgidus	CCA tRNA nucleotidyltransferase,tRNA CCA-pyrophosphorylase,tRNA adenylyl-/cytidylyl- transferase,tRNA nucleotidyltransferase,tRNA-NT
2	B, D (B, D)	Human MenBeta minihelix ending in CCACC	polymer	37	11886.1	2		Homo sapiens
3	E, M (A, C)	DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER	non-polymer		505.2	2	Chemie (APC)
4	F, N (A, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	G, H, J, O (A, B, C)	D(-)-TARTARIC ACID	non-polymer		150.1	4	Chemie (TAR)
6	I (A)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
7	K, L, P (B, D)	GUANOSINE-5'-MONOPHOSPHATE	non-polymer		363.2	3	Chemie (5GP)
8	Q, R, S, T (A, B, C, D)	water	water		18.0	215	Chemie (HOH)

Sequence modifications

A, C: 1 - 437 (UniProt: O28126)

PDB	External Database	Details
Ser 438	-	expression tag
Asn 439	-	expression tag
Ser 440	-	expression tag
Ser 441	-	expression tag
Ser 442	-	expression tag
Val 443	-	expression tag
Asp 444	-	expression tag
Lys 445	-	expression tag
Leu 446	-	expression tag
Ala 447	-	expression tag
Ala 448	-	expression tag
Ala 449	-	expression tag
Leu 450	-	expression tag
Glu 451	-	expression tag
His 452	-	expression tag
His 453	-	expression tag
His 454	-	expression tag
His 455	-	expression tag
His 456	-	expression tag
His 457	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	131117.1
Non-Polymers*	Number of molecules	12
	Total formula weight	2944.3
All*	Total formula weight	134061.4

*Water molecules are not included.