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4X4V

Crystal structure of the A.fulgidus CCA-adding enzyme in complex with a human MenBeta minihelix ending in CCACC and AMPcPP

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X25
Synchrotron siteNSLS
BeamlineX25
Temperature [K]100
Detector technologyPIXEL
Collection date2013-03-07
DetectorDECTRIS PILATUS 6M
Wavelength(s)1.1
Spacegroup nameP 21 21 2
Unit cell lengths111.059, 215.602, 58.629
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution29.680 - 2.600
R-factor0.1868
Rwork0.184
R-free0.23720
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.007
RMSD bond angle0.989
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwarePHASER
Refinement softwarePHENIX ((phenix.refine: 1.8.2_1309))
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]30.00030.0002.640
High resolution limit [Å]2.6007.0302.600
Rmerge0.0930.0400.384
Total number of observations143132
Number of reflections43940
<I/σ(I)>7.1
Completeness [%]98.596.290.9
Redundancy3.33.12.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP6.829023.2% PEG3350, 0.2M NaK tartrate, 0.1M MES pH 6.8

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