4WRN

Crystal structure of the polymerization region of human uromodulin/Tamm-Horsfall protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Maltose-binding periplasmic protein,Uromodulin	polymer	695	76539.4	2	UniProt (P0AEY0) UniProt (P07911) Pfam (PF01547) Pfam (PF00100)	Escherichia coli O157:H7	MBP,MMBP,Maltodextrin-binding protein,Tamm-Horsfall urinary glycoprotein,THP
2	C, D (C, D)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
3	E, F, I (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
4	G, H (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A, B: 25 - 391 (UniProt: P0AEY0)

PDB	External Database	Details
Glu 16	-	expression tag
Thr 17	-	expression tag
Gly 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
Thr 26	Ile 28	engineered mutation
Ala 106	Asp 108	engineered mutation
Ala 107	Lys 109	engineered mutation
Ala 196	Glu 198	engineered mutation
Ala 197	Asn 199	engineered mutation
His 239	Ala 241	engineered mutation
His 243	Lys 245	engineered mutation
Ala 263	Lys 265	engineered mutation
Val 336	Ala 338	engineered mutation
Val 341	Ile 343	engineered mutation
Ala 383	Glu 385	engineered mutation
Ala 386	Lys 388	engineered mutation
Ala 387	Asp 389	engineered mutation
Asn 391	Arg 393	engineered mutation
Ala 392	-	linker
Ala 393	-	linker
Ala 394	-	linker

A, B: 395 - 710 (UniProt: P07911)

PDB	External Database	Details
Gln 613	Asn 513	engineered mutation
Ala 686	Arg 586	engineered mutation
Ala 688	Arg 588	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	153078.8
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	5
	Total formula weight	794.4
All*	Total formula weight	154557.9

*Water molecules are not included.