4WRN
Crystal structure of the polymerization region of human uromodulin/Tamm-Horsfall protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97625 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 242.320, 242.320, 258.860 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.976 - 3.200 |
| R-factor | 0.2222 |
| Rwork | 0.221 |
| R-free | 0.24590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sex |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.840 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.314 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.088 | 1.462 |
| Number of reflections | 47837 | |
| <I/σ(I)> | 16.21 | 1.29 |
| Completeness [%] | 99.4 | 99.52 |
| Redundancy | 6.3 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293 | Sodium/potassium tartrate, Tris-HCl |
