4WQ4

E. coli YgjD(E12A)-YeaZ heterodimer in complex with ATP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	tRNA N6-adenosine threonylcarbamoyltransferase	polymer	343	36821.2	2	UniProt (P05852) Pfam (PF00814)	Escherichia coli	N6-L-threonylcarbamoyladenine synthase,t(6)A synthase,t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD,tRNA threonylcarbamoyladenosine biosynthesis protein TsaD
2	C, D (C, D)	tRNA threonylcarbamoyladenosine biosynthesis protein TsaB	polymer	237	26033.6	2	UniProt (P76256) Pfam (PF00814)	Escherichia coli	t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaB
3	E, H (A, B)	FE (III) ION	non-polymer		55.8	2	Chemie (FE)
4	F, I (A, B)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
5	G, J (A, B)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
6	K (C)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
7	L, M (C, D)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
8	N, O, P, Q (A, B, C, D)	water	water		18.0	282	Chemie (HOH)

Sequence modifications

A, B: 1 - 337 (UniProt: P05852)

PDB	External Database	Details
Ala 12	Glu 12	engineered mutation
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag
His 343	-	expression tag

C, D: 1 - 231 (UniProt: P76256)

PDB	External Database	Details
His 232	-	expression tag
His 233	-	expression tag
His 234	-	expression tag
His 235	-	expression tag
His 236	-	expression tag
His 237	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	125709.7
Non-Polymers*	Number of molecules	9
	Total formula weight	1548.5
All*	Total formula weight	127258.2

*Water molecules are not included.