4WQ4
E. coli YgjD(E12A)-YeaZ heterodimer in complex with ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 173 |
Detector technology | PIXEL |
Collection date | 2013-12-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 |
Unit cell lengths | 63.270, 68.070, 87.130 |
Unit cell angles | 109.30, 92.98, 117.23 |
Refinement procedure
Resolution | 49.040 - 2.330 |
R-factor | 0.20479 |
Rwork | 0.202 |
R-free | 0.25265 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.454 |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.330 |
Number of reflections | 49021 |
<I/σ(I)> | 11.42 |
Completeness [%] | 96.9 |
Redundancy | 3.18 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES pH7.5, 25% PEG4000, 0.3 M Lithium Sulfate, 0.1 M Sodium Acetate |