4WHV

E3 ubiquitin-protein ligase RNF8 in complex with Ubiquitin-conjugating enzyme E2 N and Polyubiquitin-B

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, E, H (B, E, H, K)	Ubiquitin-conjugating enzyme E2 N	polymer	160	17968.7	4	UniProt (P61088) Pfam (PF00179)	Homo sapiens (Human)	Bendless-like ubiquitin-conjugating enzyme,Ubc13,UbcH13,Ubiquitin carrier protein N,Ubiquitin-protein ligase N
2	B, C, F, G (C, D, I, J)	E3 ubiquitin-protein ligase RNF8	polymer	149	17646.5	4	UniProt (O76064) Pfam (PF13920)	Homo sapiens (Human)	hRNF8,RING finger protein 8
3	I, J, K, L (A, F, G, L)	Polyubiquitin-B	polymer	83	9291.6	4	UniProt (P0CG47) Pfam (PF00240)	Homo sapiens (Human)
4	M, N, O, P, Q... (C, D, I, J)	ZINC ION	non-polymer		65.4	8	Chemie (ZN)

Sequence modifications

B, E, H, K: 1 - 152 (UniProt: P61088)

PDB	External Database	Details
Gly -7	-	expression tag
Pro -6	-	expression tag
Leu -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Pro -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag
Lys 87	Cys 87	engineered mutation

C, D, I, J: 345 - 485 (UniProt: O76064)

PDB	External Database	Details
Gly 337	-	expression tag
Pro 338	-	expression tag
Leu 339	-	expression tag
Gly 340	-	expression tag
Ser 341	-	expression tag
Pro 342	-	expression tag
Glu 343	-	expression tag
Phe 344	-	expression tag

A, F, G, L: 1 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Gly -6	-	expression tag
Pro -5	-	expression tag
Gly -4	-	expression tag
Tyr -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	179626.8
Non-Polymers*	Number of molecules	8
	Total formula weight	523.3
All*	Total formula weight	180150.1

*Water molecules are not included.