4WHV

E3 ubiquitin-protein ligase RNF8 in complex with Ubiquitin-conjugating enzyme E2 N and Polyubiquitin-B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	105
Detector technology	CCD
Collection date	2013-07-19
Detector	ADSC QUANTUM 315
Wavelength(s)	1.03321
Spacegroup name	P 64 2 2
Unit cell lengths	341.379, 341.379, 113.447
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.274 - 8.300
R-factor	0.3298
Rwork	0.329
R-free	0.33740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4orh
RMSD bond length	0.007
RMSD bond angle	1.089
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	8.600
High resolution limit [Å]	8.300	17.640	8.300
Rmerge	0.191	0.055	0.901
Total number of observations	89114
Number of reflections	4094
<I/σ(I)>	6.7
Completeness [%]	100.0	100	100
Redundancy	21.8	13.3	24.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	1.04 M (NH4)2HPO4