4W8F

Crystal structure of the dynein motor domain in the AMPPNP-bound state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Dynein heavy chain lysozyme chimera	polymer	2661	304889.9	2	UniProt (P36022) UniProt (P00720) Pfam (PF08393) Pfam (PF03028) Pfam (PF18198) Pfam (PF12774) Pfam (PF21499) Pfam (PF12775) Pfam (PF22597) Pfam (PF12780) Pfam (PF12777) Pfam (PF00959) Pfam (PF12781)	Saccharomyces cerevisiae (Baker's yeast)	Dynein heavy chain,cytosolic,DYHC,Lysis protein,Muramidase
2	C, D, E, F, H... (A, B)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	8	Chemie (ANP)
3	G, L (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, B: 1364 - 3119 (UniProt: P36022)

PDB	External Database	Details
Gly 1363	-	expression tag
Gln 1849	Glu 1849	engineered mutation

A, B: 3126 - 3285 (UniProt: P00720)

PDB	External Database	Details
Gly 3136	Arg 12	conflict
Thr 3178	Cys 54	conflict
Ala 3221	Cys 97	conflict
Arg 3261	Ile 137	conflict
Gly 3286	-	linker
Ser 3287	-	linker
Gly 3288	-	linker
Ser 3289	-	linker
Gly 3290	-	linker
Ser 3291	-	linker

A, B: 3292 - 4092 (UniProt: P36022)

PDB	External Database	Details
Gly 3120	-	linker
Ser 3121	-	linker
Gly 3122	-	linker
Ser 3123	-	linker
Gly 3124	-	linker
Ser 3125	-	linker
Asp 3742	Asn 3742	conflict
Val 3895	Phe 3895	conflict
Asp 4072	Asn 4072	conflict
Gly 4093	-	linker
Ser 4094	-	linker
Gly 4095	-	linker
Ser 4096	-	linker
Gly 4097	-	linker
Ser 4098	-	linker
His 4099	-	expression tag
His 4100	-	expression tag
His 4101	-	expression tag
His 4102	-	expression tag
His 4103	-	expression tag
His 4104	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	609779.8
Non-Polymers*	Number of molecules	10
	Total formula weight	4098.2
All*	Total formula weight	613877.9

*Water molecules are not included.