4W8F
Crystal structure of the dynein motor domain in the AMPPNP-bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1159 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 137.730, 154.380, 177.550 |
| Unit cell angles | 90.00, 96.59, 90.00 |
Refinement procedure
| Resolution | 49.400 - 3.541 |
| R-factor | 0.2299 |
| Rwork | 0.228 |
| R-free | 0.26200 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.149 |
| Refinement software | PHENIX ((phenix.refine: dev_1769)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.400 |
| High resolution limit [Å] | 3.540 |
| Number of reflections | 82983 |
| <I/σ(I)> | 8.9 |
| Completeness [%] | 92.2 |
| Redundancy | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 4-10% PEG 3350 and 200-300 mM NaAc |
