4UYA

Structure of MLK4 kinase domain with ATPgammaS

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE MLK4	polymer	340	38473.2	1	UniProt (Q5TCX8) Pfam (PF07714)	HOMO SAPIENS (HUMAN)	MIXED LINEAGE KINASE 4
2	B (A)	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	1	Chemie (AGS)
3	C, D (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	E (A)	water	water		18.0	39	Chemie (HOH)

Sequence modifications

A: 115 - 451 (UniProt: Q5TCX8)

PDB	External Database	Details
Met 97	-	expression tag
Gly 98	-	expression tag
His 99	-	expression tag
His 100	-	expression tag
His 101	-	expression tag
His 102	-	expression tag
His 103	-	expression tag
His 104	-	expression tag
Gly 105	-	expression tag
Gly 106	-	expression tag
Gly 107	-	expression tag
Glu 108	-	expression tag
Asn 109	-	expression tag
Leu 110	-	expression tag
Tyr 111	-	expression tag
Phe 112	-	expression tag
Gln 113	-	expression tag
Gly 114	-	expression tag
Gly 214	Ala 214	engineered mutation
-	Ala 215	deletion
-	Asn 216	deletion
-	Ala 217	deletion
-	Ala 218	deletion
-	Pro 219	deletion
-	Asp 220	deletion
-	Pro 221	deletion
-	Arg 222	deletion
-	Ala 223	deletion
-	Pro 224	deletion
-	Gly 225	deletion
-	Pro 226	deletion
-	Arg 227	deletion
-	Arg 228	deletion
-	Ala 229	deletion
Ala 303	Ser 303	engineered mutation
Ala 307	Thr 307	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	38473.2
Non-Polymers*	Number of molecules	3
	Total formula weight	571.9
All*	Total formula weight	39045.1

*Water molecules are not included.