4UTW

Structural characterisation of NanE, ManNac6P C2 epimerase, from Clostridium perfingens

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	PUTATIVE N-ACETYLMANNOSAMINE-6-PHOSPHATE 2-EPIMERASE	polymer	229	25164.9	2	UniProt (Q0TUP9) Pfam (PF04131) UniProt (by SIFTS) (Q8XNZ3)	CLOSTRIDIUM PERFRINGENS STR. 13	MANNAC-6-P EPIMERASE, N-ACETYLMANNOSAMINE-6-PHOSPHATE 2-EPIMERASE
2	C, D (C, D)	PUTATIVE N-ACETYLMANNOSAMINE-6-PHOSPHATE 2-EPIMERASE	polymer	229	25113.8	2	UniProt (Q0TUP9) Pfam (PF04131) UniProt (by SIFTS) (Q8XNZ3)	CLOSTRIDIUM PERFRINGENS STR. 13	MANNAC-6-P EPIMERASE, N-ACETYLMANNOSAMINE-6-PHOSPHATE 2-EPIMERASE
3	E, G, I, K (A, B, C, D)	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
4	F, H, J, L (A, B, C, D)	N-acetyl-D-glucosamine-6-phosphate	non-polymer		299.2	4	Chemie (RFW)
5	M, N, O, P (A, B, C, D)	water	water		18.0	999	Chemie (HOH)

Sequence modifications

A, B: 1 - 220 (UniProt: Q0TUP9)

PDB	External Database	Details
Gly -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

C, D: 1 - 220 (UniProt: Q0TUP9)

PDB	External Database	Details
Gly -8	-	expression tag
Ser -7	-	expression tag
Ser -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	100557.5
Non-Polymers*	Number of molecules	8
	Total formula weight	1338.5
All*	Total formula weight	101896.0

*Water molecules are not included.