4UN5
Crystal structure of Cas9 bound to PAM-containing DNA target containing mismatches at positions 1-3
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | SGRNA | polymer | 83 | 26789.0 | 1 | SYNTHETIC CONSTRUCT | ||
| 2 | B | CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 | polymer | 1372 | 158875.0 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) In PDB | STREPTOCOCCUS PYOGENES | |
| 3 | C | TARGET DNA STRAND PROXIMAL FRAGMENT | polymer | 11 | 3295.2 | 1 | SYNTHETIC CONSTRUCT | ||
| 4 | D | NON-TARGET DNA STRAND | polymer | 11 | 3410.2 | 1 | SYNTHETIC CONSTRUCT | ||
| 5 | E | TARGET DNA STRAND DISTAL FRAGMENT | polymer | 17 | 5215.4 | 1 | SYNTHETIC CONSTRUCT | ||
| 6 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 8 | Chemie (MG) | |||
| 7 | water | water | 18.0 | 772 | Chemie (HOH) |
Sequence modifications
B: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ala 10 | Asp 10 | engineered mutation |
| Ala 840 | His 840 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 197584.9 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 194.4 | |
| All* | Total formula weight | 197779.3 |
