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4UN5

Crystal structure of Cas9 bound to PAM-containing DNA target containing mismatches at positions 1-3

Functional Information from GO Data
ChainGOidnamespacecontents
B0003677molecular_functionDNA binding
B0003723molecular_functionRNA binding
B0004519molecular_functionendonuclease activity
B0004520molecular_functionDNA endonuclease activity
B0004527molecular_functionexonuclease activity
B0008408molecular_function3'-5' exonuclease activity
B0043571biological_processmaintenance of CRISPR repeat elements
B0046872molecular_functionmetal ion binding
B0051607biological_processdefense response to virus
Functional Information from PDB Data
site_idAC1
Number of Residues5
DetailsBINDING SITE FOR RESIDUE MG A 1082
ChainResidue
AU50
AHOH2144
AHOH2145
AHOH2146
AHOH2162

site_idAC2
Number of Residues5
DetailsBINDING SITE FOR RESIDUE MG A 1083
ChainResidue
BTHR1102
AA65
AU66
AHOH2202
AHOH2214

site_idAC3
Number of Residues3
DetailsBINDING SITE FOR RESIDUE MG A 1084
ChainResidue
AHOH2082
AHOH2084
AHOH2233

site_idAC4
Number of Residues4
DetailsBINDING SITE FOR RESIDUE MG A 1085
ChainResidue
AG21
AHOH2080
AHOH2082
AHOH2121

site_idAC5
Number of Residues5
DetailsBINDING SITE FOR RESIDUE MG B 2365
ChainResidue
BLEU623
BTHR624
BPHE626
BTYR656
BHOH3261

site_idAC6
Number of Residues4
DetailsBINDING SITE FOR RESIDUE MG B 2366
ChainResidue
AA68
AHOH2215
BGLN1350
BHOH3489

site_idAC7
Number of Residues3
DetailsBINDING SITE FOR RESIDUE MG B 2367
ChainResidue
BILE226
BLEU229
BGLU232

site_idAC8
Number of Residues3
DetailsBINDING SITE FOR RESIDUE MG B 2368
ChainResidue
BGLU584
BARG586
BASN588

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues1
DetailsACT_SITE: For RuvC-like nuclease domain => ECO:0000305|PubMed:24529477
ChainResidueDetails
BALA10

site_idSWS_FT_FI2
Number of Residues1
DetailsACT_SITE: Proton acceptor for HNH nuclease domain => ECO:0000305|PubMed:24529477
ChainResidueDetails
BALA840

site_idSWS_FT_FI3
Number of Residues6
DetailsBINDING: BINDING => ECO:0000269|PubMed:24505130
ChainResidueDetails
BALA10
BGLU762
BGLU766
BHIS983
BHIS1297
BASP1328

227344

PDB entries from 2024-11-13

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