4UN5
Crystal structure of Cas9 bound to PAM-containing DNA target containing mismatches at positions 1-3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-10 |
Detector | DECTRIS PILATUS |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 180.182, 68.072, 190.261 |
Unit cell angles | 90.00, 111.37, 90.00 |
Refinement procedure
Resolution | 48.349 - 2.400 |
R-factor | 0.2165 |
Rwork | 0.215 |
R-free | 0.24650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4un3 |
RMSD bond length | 0.011 |
RMSD bond angle | 0.686 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.350 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.090 | 0.370 |
Number of reflections | 164018 | |
<I/σ(I)> | 15.3 | 4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.6 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 0.1 M TRIS-ACETATE PH 8.5, 0.3 M KSCN, 15% PEG (W/V) 3350 |