4UMM
The Cryo-EM structure of the palindromic DNA-bound USP-EcR nuclear receptor reveals an asymmetric organization with allosteric domain positioning
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | ECR-USP | polymer | 78 | 9256.8 | 1 | HELIOTHIS VIRESCENS (TOBACCO BUDWORM) | ||
| 2 | B (C) | 5'-D(*CP*AP*AP*GP*GP*GP*TP*TP*CP*AP*AP*TP*GP*CP *AP*CP*TP*TP*GP*TP)-3' | polymer | 20 | 6149.0 | 1 | SYNTHETIC CONSTRUCT | ||
| 3 | C (D) | 5'-D(*DGP*AP*CP*AP*AP*GP*TP*GP*CP*AP*TP*TP*GP*DAP *AP*CP*CP*CP*TP*T)-3' | polymer | 20 | 6118.0 | 1 | SYNTHETIC CONSTRUCT | ||
| 4 | D (E) | ECDYSONE RECEPTOR | polymer | 87 | 10100.0 | 1 | UniProt (O18473) Pfam (PF00105) | HELIOTHIS VIRESCENS | 20-HYDROXY-ECDYSONE RECEPTOR, 20E RECEPTOR, ECRH, ECDYSTEROID RECEPTOR, HVECR, NUCLEAR RECEPTOR SUBFAMILY 1 GROUP H MEMBER 1 |
| 5 | E (F) | GENE REGULATION PROTEIN | polymer | 264 | 29962.7 | 1 | UniProt (Q7SIF6) Pfam (PF00104) | HELIOTHIS VIRESCENS (TOBACCO BUDWORM) | ECR-USP |
| 6 | F (G) | ECDYSONE RECEPTOR | polymer | 266 | 30369.0 | 1 | UniProt (O18473) Pfam (PF00104) | HELIOTHIS VIRESCENS (TOBACCO BUDWORM) | 20-HYDROXY-ECDYSONE RECEPTOR, 20E RECEPTOR, ECRH, ECDYSTEROID RECEPTOR, HVECR, NUCLEAR RECEPTOR SUBFAMILY 1 GROUP H MEMBER 1 |
| 7 | G (F) | L-ALPHA-PHOSPHATIDYL-BETA-OLEOYL-GAMMA-PALMITOYL-PHOSPHATIDYLETHANOLAMINE | non-polymer | 709.9 | 1 | Chemie (EPH) | |||
| 8 | H (G) | 2,3,14,20,22-PENTAHYDROXYCHOLEST-7-EN-6-ONE | non-polymer | 464.6 | 1 | Chemie (P1A) | |||
| 9 | I, J, K, L, M (F, G) | water | water | 18.0 | 8 | Chemie (HOH) |
Sequence modifications
E: 141 - 227 (UniProt: O18473)
G: 284 - 532 (UniProt: O18473)
| PDB | External Database | Details |
|---|---|---|
| Met 193 | Ile 209 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Gly 267 | - | expression tag |
| Ser 268 | - | expression tag |
| His 269 | - | expression tag |
| Met 270 | - | expression tag |
| Ala 271 | - | expression tag |
| Ser 272 | - | expression tag |
| Met 273 | - | expression tag |
| Thr 274 | - | expression tag |
| Gly 275 | - | expression tag |
| Gly 276 | - | expression tag |
| Gln 277 | - | expression tag |
| Gln 278 | - | expression tag |
| Met 279 | - | expression tag |
| Gly 280 | - | expression tag |
| Arg 281 | - | expression tag |
| Asp 282 | - | expression tag |
| Pro 283 | - | expression tag |
| Thr 322 | - | insertion |
| Trp 323 | - | insertion |
| Gln 324 | - | insertion |
| Cys 483 | Gly 501 | conflict |
| Lys 489 | Glu 507 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 91955.4 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 1174.6 | |
| All* | Total formula weight | 93130.0 |
