4UMM
The Cryo-EM structure of the palindromic DNA-bound USP-EcR nuclear receptor reveals an asymmetric organization with allosteric domain positioning
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | ECR-USP | polymer | 78 | 9256.8 | 1 | HELIOTHIS VIRESCENS (TOBACCO BUDWORM) | ||
| 2 | B (C) | 5'-D(*CP*AP*AP*GP*GP*GP*TP*TP*CP*AP*AP*TP*GP*CP *AP*CP*TP*TP*GP*TP)-3' | polymer | 20 | 6149.0 | 1 | SYNTHETIC CONSTRUCT | ||
| 3 | C (D) | 5'-D(*DGP*AP*CP*AP*AP*GP*TP*GP*CP*AP*TP*TP*GP*DAP *AP*CP*CP*CP*TP*T)-3' | polymer | 20 | 6118.0 | 1 | SYNTHETIC CONSTRUCT | ||
| 4 | D (E) | ECDYSONE RECEPTOR | polymer | 87 | 10100.0 | 1 | UniProt (O18473) | HELIOTHIS VIRESCENS | 20-HYDROXY-ECDYSONE RECEPTOR, 20E RECEPTOR, ECRH, ECDYSTEROID RECEPTOR, HVECR, NUCLEAR RECEPTOR SUBFAMILY 1 GROUP H MEMBER 1 |
| 5 | E (F) | GENE REGULATION PROTEIN | polymer | 264 | 29962.7 | 1 | UniProt (Q7SIF6) | HELIOTHIS VIRESCENS (TOBACCO BUDWORM) | ECR-USP |
| 6 | F (G) | ECDYSONE RECEPTOR | polymer | 266 | 30369.0 | 1 | UniProt (O18473) | HELIOTHIS VIRESCENS (TOBACCO BUDWORM) | 20-HYDROXY-ECDYSONE RECEPTOR, 20E RECEPTOR, ECRH, ECDYSTEROID RECEPTOR, HVECR, NUCLEAR RECEPTOR SUBFAMILY 1 GROUP H MEMBER 1 |
| 7 | G (F) | L-ALPHA-PHOSPHATIDYL-BETA-OLEOYL-GAMMA-PALMITOYL-PHOSPHATIDYLETHANOLAMINE | non-polymer | 709.9 | 1 | Chemie (EPH) PubChem (46936564) PubChem (4474776) PubChem (5288167) PubChem (6323300) PubChem (168313614) | |||
| 8 | H (G) | 2,3,14,20,22-PENTAHYDROXYCHOLEST-7-EN-6-ONE | non-polymer | 464.6 | 1 | Chemie (P1A) PubChem (21119315) PubChem (24985076) PubChem (177677459) PubChem (44143975) PubChem (44231486) PubChem (21119316) PubChem (115127) PubChem (57988722) PubChem (73758207) PubChem (4482619) PubChem (5320672) PubChem (91884703) PubChem (6325829) PubChem (12314451) PubChem (138988226) PubChem (11984598) PubChem (129664321) PubChem (146674585) PubChem (154731155) PubChem (154790218) PubChem (160227469) PubChem (163096526) PubChem (163096527) PubChem (163096528) PubChem (163096529) PubChem (163096530) PubChem (163342445) PubChem (163869997) PubChem (170325868) | |||
| 9 | I, J, K, L, M (F, G) | water | water | 18.0 | 8 | Chemie (HOH) |
Sequence modifications
E: 141 - 227 (UniProt: O18473)
G: 284 - 532 (UniProt: O18473)
| PDB | External Database | Details |
|---|---|---|
| Met 193 | Ile 209 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Gly 267 | - | expression tag |
| Ser 268 | - | expression tag |
| His 269 | - | expression tag |
| Met 270 | - | expression tag |
| Ala 271 | - | expression tag |
| Ser 272 | - | expression tag |
| Met 273 | - | expression tag |
| Thr 274 | - | expression tag |
| Gly 275 | - | expression tag |
| Gly 276 | - | expression tag |
| Gln 277 | - | expression tag |
| Gln 278 | - | expression tag |
| Met 279 | - | expression tag |
| Gly 280 | - | expression tag |
| Arg 281 | - | expression tag |
| Asp 282 | - | expression tag |
| Pro 283 | - | expression tag |
| Thr 322 | - | insertion |
| Trp 323 | - | insertion |
| Gln 324 | - | insertion |
| Cys 483 | Gly 501 | conflict |
| Lys 489 | Glu 507 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 91955.4 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 1174.6 | |
| All* | Total formula weight | 93130.0 |
