4U5W

Crystal Structure of HIV-1 Nef-SF2 Core Domain in Complex with the Src Family Kinase Hck SH3-SH2 Tandem Regulatory Domains

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Protein Nef	polymer	149	17592.9	2	UniProt (P03407) Pfam (PF00469) In PDB	Human immunodeficiency virus type 1 (HIV-1)	3'ORF,Negative factor,F-protein
2	B, D	Tyrosine-protein kinase HCK	polymer	180	20742.2	2	UniProt (P08631) Pfam (PF00018) Pfam (PF00017) In PDB	Homo sapiens (Human)	Hematopoietic cell kinase,Hemopoietic cell kinase,p59-HCK/p60-HCK,p59Hck,p61Hck
3	A	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	3	Chemie (MPD)
4	A, B, C, D	IODIDE ION	non-polymer		126.9	6	Chemie (IOD)
5		water	water		18.0	525	Chemie (HOH)

Sequence modifications

A, C: 62 - 209 (UniProt: P03407)

PDB	External Database	Details
Met 61	-	initiating methionine

B, D: 75 - 246 (UniProt: P08631)

PDB	External Database	Details
Met 74	-	initiating methionine
Leu 247	-	expression tag
Glu 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag
His 252	-	expression tag
His 253	-	expression tag
His 254	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	76670.3
Non-Polymers*	Number of molecules	9
	Total formula weight	1115.9
All*	Total formula weight	77786.3

*Water molecules are not included.