4U5W

Crystal Structure of HIV-1 Nef-SF2 Core Domain in Complex with the Src Family Kinase Hck SH3-SH2 Tandem Regulatory Domains

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005525	molecular_function	GTP binding
C	0005525	molecular_function	GTP binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue MPD A 301

Chain	Residue
A	ARG81
A	HOH452
B	ASP99
B	TYR131

---

site_id	AC2
Number of Residues	8
Details	binding site for residue MPD A 302

Chain	Residue
B	ASN135
B	HOH416
D	GLU225
A	GLN77
A	VAL78
A	PRO79
A	HOH440
A	HOH442

---

site_id	AC3
Number of Residues	4
Details	binding site for residue MPD A 303

Chain	Residue
A	ARG110
A	ILE113
C	LEU104
C	ARG110

---

site_id	AC4
Number of Residues	4
Details	binding site for residue IOD A 304

Chain	Residue
A	PRO82
D	THR219
D	IOD302
D	HOH435

---

site_id	AC5
Number of Residues	1
Details	binding site for residue IOD B 301

Chain	Residue
B	GLN224

---

site_id	AC6
Number of Residues	2
Details	binding site for residue IOD C 301

Chain	Residue
C	LEU80
C	PRO82

---

site_id	AC7
Number of Residues	1
Details	binding site for residue IOD D 301

Chain	Residue
D	LYS231

---

site_id	AC8
Number of Residues	5
Details	binding site for residue IOD D 302

Chain	Residue
A	IOD304
A	HOH443
D	LEU207
D	ASN209
D	HOH424

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	18
Details	Region: {"description":"SH3-binding; interaction with Src family tyrosine kinases","evidences":[{"source":"HAMAP-Rule","id":"MF_04078","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	32
Details	Region: {"description":"Mediates dimerization, Nef-PTE1 interaction","evidences":[{"source":"HAMAP-Rule","id":"MF_04078","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	Motif: {"description":"PxxP; stabilizes the interaction of NEF/MHC-I with host AP1M1; necessary for MHC-I internalization","evidences":[{"source":"HAMAP-Rule","id":"MF_04078","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	97
Details	Domain: {"description":"SH2","evidences":[{"source":"PROSITE-ProRule","id":"PRU00191","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"19369195","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"P08103","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---