4TU9

STRUCTURE OF U2AF65 VARIANT WITH BRU5G6 DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Splicing factor U2AF 65 kDa subunit	polymer	174	19075.8	2	UniProt (P26368) Pfam (PF00076)	Homo sapiens (Human)	U2 auxiliary factor 65 kDa subunit,hU2AF65,U2 snRNP auxiliary factor large subunit
2	C, D (P, E)	DNA (5'-D(*UP*UP*UP*UP*(BRU)P*DG*U)-3')	polymer	7	2104.1	2		Homo sapiens
3	E, F, G, L, M (A, B)	1,4-DIETHYLENE DIOXIDE	non-polymer		88.1	5	Chemie (DIO)
4	H, I, J, O, P... (A, B)	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)
5	K, R (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6	N (B)	N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE	non-polymer		862.1	1	Chemie (CPQ)
7	S, T, U, V (A, B, P, E)	water	water		18.0	265	Chemie (HOH)

Sequence modifications

A, B: 148 - 336 (UniProt: P26368)

PDB	External Database	Details
Gly 143	-	expression tag
Pro 144	-	expression tag
Leu 145	-	expression tag
Gly 146	-	expression tag
Ser 147	-	expression tag
-	Met 238	deletion
-	Ser 239	deletion
-	Glu 240	deletion
-	Asn 241	deletion
-	Pro 242	deletion
-	Ser 243	deletion
-	Val 244	deletion
-	Tyr 245	deletion
-	Val 246	deletion
-	Pro 247	deletion
-	Gly 248	deletion
-	Val 249	deletion
-	Val 250	deletion
-	Ser 251	deletion
-	Thr 252	deletion
-	Val 253	deletion
-	Val 254	deletion
-	Pro 255	deletion
-	Asp 256	deletion
-	Ser 257	deletion

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	42359.8
Non-Polymers*	Number of molecules	14
	Total formula weight	2063.1
All*	Total formula weight	44422.9

*Water molecules are not included.