4TU9

STRUCTURE OF U2AF65 VARIANT WITH BRU5G6 DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-13
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.920
Spacegroup name	C 1 2 1
Unit cell lengths	164.488, 37.183, 101.047
Unit cell angles	90.00, 125.57, 90.00

Refinement procedure

Resolution	29.069 - 1.992
R-factor	0.1922
Rwork	0.188
R-free	0.23420
RMSD bond length	0.007
RMSD bond angle	1.205
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.030
High resolution limit [Å]	1.992	5.420	1.992
Rmerge		0.026	0.445
Number of reflections	33992
<I/σ(I)>	18.421
Completeness [%]	99.1	96.8	98.7
Redundancy	2.6	2.5	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	1.6M AMMONIUM SULFATE, 10% DIOXANE, 0.1M MES PH 6.5, DEOXY-BIG CHAP