4S26

Crystal structure of Arabidopsis thaliana ThiC with bound imidazole ribonucleotide, S-adenosylhomocysteine, Fe4S4 cluster and Zn (monoclinic crystal form)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Phosphomethylpyrimidine synthase, chloroplastic	polymer	576	64670.1	2	UniProt (O82392) Pfam (PF01964) In PDB	Arabidopsis thaliana (mouse-ear cress thale-cress)	Hydroxymethylpyrimidine phosphate synthase, HMP-P synthase, HMP-phosphate synthase, HMPP synthase, Protein PYRIMIDINE REQUIRING, Thiamine biosynthesis protein ThiC, Protein THIAMINE C
2	A, B	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)
3	A, B	1-(5-O-phosphono-beta-D-ribofuranosyl)-1H-imidazole	non-polymer		280.2	2	Chemie (IRN)
4	A, B	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)
5	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
6	A, B	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
7		water	water		18.0	544	Chemie (HOH)

Sequence modifications

A, B: 72 - 644 (UniProt: O82392)

PDB	External Database	Details
Gly 69	-	expression tag
His 70	-	expression tag
Met 71	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	129340.2
Non-Polymers*	Number of molecules	10
	Total formula weight	2234.2
All*	Total formula weight	131574.4

*Water molecules are not included.