4RWF

Crystal structure of the CLR:RAMP2 extracellular domain heterodimer with bound adrenomedullin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Maltose transporter subunit, Receptor activity-modifying protein 2, Calcitonin gene-related peptide type 1 receptor fusion protein	polymer	591	66308.4	1	UniProt (P0AEX9) UniProt (O60895) UniProt (Q16602) Pfam (PF01547) Pfam (PF04901) Pfam (PF02793)	Escherichia coli (human)
2	B (B)	Adrenomedullin	polymer	29	3252.7	1	UniProt (P35318)	Homo sapiens	CGRP type 1 receptor, Calcitonin receptor-like receptor
3	C (C)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
4	D, E, F, G, H (A)	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
5	I, J (A, B)	water	water		18.0	394	Chemie (HOH)

Sequence modifications

A: 2 - 374 (UniProt: P0AEX9)

PDB	External Database	Details
Met 1	-	expression tag

A: 1055 - 1138 (UniProt: O60895)

PDB	External Database	Details
Arg 1106	Leu 106	conflict
Ser 1139	-	linker

A: 2031 - 2116 (UniProt: Q16602)

PDB	External Database	Details
Asp 2019	-	expression tag
Gly 2020	-	expression tag
Ser 2021	-	expression tag
Ala 2022	-	expression tag
Gly 2023	-	expression tag
Ser 2024	-	expression tag
Ala 2025	-	expression tag
Gly 2026	-	expression tag
Ser 2027	-	expression tag
Ala 2028	-	expression tag
Glu 2029	-	expression tag
Asp 2030	-	expression tag

B: 25 - 52 (UniProt: P35318)

PDB	External Database	Details
Nh2 53	-	amidation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	69561.0
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	5
	Total formula weight	310.3
All*	Total formula weight	70213.6

*Water molecules are not included.