4RWF
Crystal structure of the CLR:RAMP2 extracellular domain heterodimer with bound adrenomedullin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2014-08-02 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.454, 84.283, 115.764 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.760 |
R-factor | 0.159 |
Rwork | 0.157 |
R-free | 0.20030 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.020 |
RMSD bond angle | 1.929 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.790 |
High resolution limit [Å] | 1.760 | 4.780 | 1.760 |
Rmerge | 0.068 | 0.039 | 0.966 |
Number of reflections | 70048 | ||
<I/σ(I)> | 8.3 | ||
Completeness [%] | 99.9 | 99.7 | 98.2 |
Redundancy | 7.2 | 6.9 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 293 | 19% PEG3350 0.1 M Tris-HCl, pH 8.3 225 mM Sodium Acetate 20% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |