4RIB

FAN1 Nuclease bound to 5' phosphorylated p(dT) single flap DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Fanconi-associated nuclease 1	polymer	651	73926.0	2	UniProt (Q9Y2M0) Pfam (PF21315) Pfam (PF21169) Pfam (PF21170) Pfam (PF08774)	Homo sapiens (human)	FANCD2/FANCI-associated nuclease 1, Myotubularin-related protein 15
2	C, D (X, U)	DNA (5'-D(P*TP*AP*GP*CP*CP*AP*CP*GP*CP*CP*TP*AP*GP*AP*CP*TP*CP*CP*TP*C)-3')	polymer	20	6014.9	2
3	E, F (Y, V)	DNA (5'-D(*GP*CP*TP*GP*AP*GP*GP*AP*GP*TP*CP*T)-3')	polymer	12	3718.4	2
4	G, H (Z, W)	DNA (5'-D(*TP*TP*TP*TP*TP*TP*GP*AP*GP*GP*CP*GP*TP*G)-3')	polymer	14	4332.8	2
5	I, J (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)

Sequence modifications

A, B: 364 - 1017 (UniProt: Q9Y2M0)

PDB	External Database	Details
Gly 358	-	expression tag
Ala 359	-	expression tag
His 360	-	expression tag
Met 361	-	expression tag
Thr 362	-	expression tag
Arg 363	-	expression tag
Ala 487	Val 487	engineered mutation
-	Cys 510	deletion
-	Thr 511	deletion
-	Trp 512	deletion
-	Gly 513	deletion
-	Lys 514	deletion
-	Asn 515	deletion
-	Lys 516	deletion
-	Pro 517	deletion
-	Gly 518	deletion

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	175984.3
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	176064.5

*Water molecules are not included.