4RIB
FAN1 Nuclease bound to 5' phosphorylated p(dT) single flap DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97925 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 97.697, 110.989, 105.427 |
Unit cell angles | 90.00, 103.75, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.250 |
R-factor | 0.22744 |
Rwork | 0.226 |
R-free | 0.26526 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.193 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 70.000 | 70.000 | 3.370 |
High resolution limit [Å] | 3.250 | 7.000 | 3.250 |
Rmerge | 0.114 | 0.044 | 0.704 |
Number of reflections | 32799 | ||
<I/σ(I)> | 6.1 | ||
Completeness [%] | 98.8 | 97.4 | 98.1 |
Redundancy | 5 | 5 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop vapor diffusion | 7 | 277 | PEG 3350, NaCl, pH 7.0, hanging drop vapor diffusion, temperature 277K |