4RHL

Crystal structure of T. brucei arginase-like protein triple mutant S149D/R151H/S226D bound with Mn2+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Arginase	polymer	351	38769.2	2	UniProt (Q581Y0) Pfam (PF00491)	Trypanosoma brucei brucei
2	C, E (A, B)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
3	D, F (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	G, H (A, B)	water	water		18.0	31	Chemie (HOH)

Sequence modifications

A, B: 1 - 331 (UniProt: Q581Y0)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asp 149	Ser 149	engineered mutation
His 151	Arg 151	engineered mutation
Asp 226	Ser 226	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	77538.4
Non-Polymers*	Number of molecules	4
	Total formula weight	294.1
All*	Total formula weight	77832.5

*Water molecules are not included.