4RHL

Crystal structure of T. brucei arginase-like protein triple mutant S149D/R151H/S226D bound with Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 3 2 1
Unit cell lengths	135.582, 135.582, 86.680
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.609 - 3.100
R-factor	0.218
Rwork	0.215
R-free	0.27440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rhk
RMSD bond length	0.004
RMSD bond angle	0.824
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.210
High resolution limit [Å]	3.100	6.670	3.100
Number of reflections	16959
Completeness [%]	100.0	100	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	294	20% (v/v) Jeffamine ED-2001, 0.1 M imidazole, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 294K