4QTU

Structure of S. cerevisiae Bud23-Trm112 complex involved in formation of m7G1575 on 18S rRNA (SAM bound form)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Multifunctional methyltransferase subunit TRM112	polymer	135	15078.2	2	UniProt (P53738) Pfam (PF03966) In PDB	Saccharomyces cerevisiae S288c (yeast)	rRNA MTase activator subunit, eRF1 methyltransferase subunit TRM112, eRF1 MTase subunit TRM112, tRNA methyltransferase 112
2	B, D	Putative methyltransferase BUD23	polymer	208	23048.1	2	UniProt (P25627) Pfam (PF08241) In PDB	Saccharomyces cerevisiae S288c (yeast)	rRNA MTase catalytic subunit, Bud site selection protein 23
3	A, C	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	D, A, B, C	1,2-ETHANEDIOL	non-polymer		62.1	19	Chemie (EDO)
5	B, D	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM)
6		water	water		18.0	173	Chemie (HOH)

Sequence modifications

B, D: 1 - 202 (UniProt: P25627)

PDB	External Database	Details
His 203	-	expression tag
His 204	-	expression tag
His 205	-	expression tag
His 206	-	expression tag
His 207	-	expression tag
His 208	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	76252.5
Non-Polymers*	Number of molecules	23
	Total formula weight	2107.0
All*	Total formula weight	78359.5

*Water molecules are not included.