4QTT

Structure of S. cerevisiae Bud23-Trm112 complex involved in formation of m7G1575 on 18S rRNA (apo-form)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Multifunctional methyltransferase subunit TRM112	polymer	135	15078.2	2	UniProt (P53738) Pfam (PF03966)	Saccharomyces cerevisiae S288c (yeast)	rRNA MTase activator subunit, eRF1 methyltransferase subunit TRM112, eRF1 MTase subunit TRM112, tRNA methyltransferase 112
2	B, D (B, D)	Putative methyltransferase BUD23	polymer	208	23048.1	2	UniProt (P25627) Pfam (PF08241)	Saccharomyces cerevisiae S288c (yeast)	rRNA MTase catalytic subunit, Bud site selection protein 23
3	E, K (A, C)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	F, G, H, I (B)	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
5	J (B)	DODECAETHYLENE GLYCOL	non-polymer		546.6	1	Chemie (12P)
6	L, M, N, O (A, B, C, D)	water	water		18.0	270	Chemie (HOH)

Sequence modifications

B, D: 1 - 202 (UniProt: P25627)

PDB	External Database	Details
His 203	-	expression tag
His 204	-	expression tag
His 205	-	expression tag
His 206	-	expression tag
His 207	-	expression tag
His 208	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	76252.5
Non-Polymers*	Number of molecules	7
	Total formula weight	925.7
All*	Total formula weight	77178.3

*Water molecules are not included.