4QT8

Crystal Structure of RON Sema-PSI-IPT1 extracellular domains in complex with MSP beta-chain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, A)	Macrophage-stimulating protein receptor	polymer	669	71987.4	2	UniProt (Q04912) Pfam (PF01403) Pfam (PF01437) Pfam (PF01833)	Homo sapiens (human)	MSP receptor, CDw136, Protein-tyrosine kinase 8, p185-Ron, RON receptor tyrosine kinase
2	C, D (C, D)	Hepatocyte growth factor-like protein	polymer	253	28336.7	2	UniProt (P26927) Pfam (PF00089)	Homo sapiens (human)	Macrophage stimulatory protein, Macrophage-stimulating protein, MSP
3	E (E)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
4	F (F)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)

Sequence modifications

B, A: 25 - 683 (UniProt: Q04912)

PDB	External Database	Details
Arg 23	-	expression tag
Ser 24	-	expression tag
Gly 209	Ala 209	conflict
Leu 306	Arg 306	engineered mutation
Val 307	Arg 307	engineered mutation
Pro 308	Arg 308	engineered mutation
Ser 311	Ala 311	engineered mutation
Gln 322	Arg 322	engineered mutation
Thr 684	-	expression tag
Gly 685	-	expression tag
His 686	-	expression tag
His 687	-	expression tag
His 688	-	expression tag
His 689	-	expression tag
His 690	-	expression tag
His 691	-	expression tag

C, D: 465 - 711 (UniProt: P26927)

PDB	External Database	Details
Ser 672	Cys 672	engineered mutation
His 712	-	expression tag
His 713	-	expression tag
His 714	-	expression tag
His 715	-	expression tag
His 716	-	expression tag
His 717	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	200648.4
Branched	Number of molecules	2
	Total formula weight	1010.9
All*	Total formula weight	201659.3

*Water molecules are not included.