4QT8
Crystal Structure of RON Sema-PSI-IPT1 extracellular domains in complex with MSP beta-chain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03320 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 106.585, 63.775, 146.049 |
Unit cell angles | 90.00, 90.08, 90.00 |
Refinement procedure
Resolution | 19.980 - 3.000 |
R-factor | 0.23677 |
Rwork | 0.234 |
R-free | 0.29077 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 4FWW & 2ASU |
RMSD bond length | 0.009 |
RMSD bond angle | 1.391 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 146.960 | |
High resolution limit [Å] | 2.996 | 2.996 |
Rmerge | 0.202 | |
Number of reflections | 38960 | |
<I/σ(I)> | 4.4 | |
Completeness [%] | 91.8 | |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.1 M Tris-HCl, pH 8.5, 20% w/v PEG4000, 8% v/v isopropanol, 4% v/v polypropylene glycol 400, VAPOR DIFFUSION, SITTING DROP, temperature 298K |