4QPL

Crystal structure of RNF146(RING-WWE)/UbcH5a/iso-ADPr complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	E3 ubiquitin-protein ligase RNF146	polymer	155	17857.6	2	UniProt (Q9CZW6) Pfam (PF13920) Pfam (PF02825)	Mus musculus (mouse)	Iduna, RING finger protein 146
2	B, D (B, D)	Ubiquitin-conjugating enzyme E2 D1	polymer	154	17522.0	2	UniProt (P51668) Pfam (PF00179)	Homo sapiens (human)	Stimulator of Fe transport, SFT, UBC4/5 homolog, UbcH5, Ubiquitin carrier protein D1, Ubiquitin-conjugating enzyme E2(17)KB 1, Ubiquitin-conjugating enzyme E2-17 kDa 1, Ubiquitin-protein ligase D1
3	E, H (A, C)	2'-O-(5-O-phosphono-alpha-D-ribofuranosyl)adenosine 5'-(dihydrogen phosphate)	non-polymer		559.3	2	Chemie (V3L)
4	F, G, I, J (A, C)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5	K, L, M, N (A, B, C, D)	water	water		18.0	371	Chemie (HOH)

Sequence modifications

A, C: 30 - 183 (UniProt: Q9CZW6)

PDB	External Database	Details
Gly 29	-	expression tag

B, D: 1 - 147 (UniProt: P51668)

PDB	External Database	Details
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	70759.1
Non-Polymers*	Number of molecules	6
	Total formula weight	1380.3
All*	Total formula weight	72139.4

*Water molecules are not included.