4QPL

Crystal structure of RNF146(RING-WWE)/UbcH5a/iso-ADPr complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0008270	molecular_function	zinc ion binding
A	0016055	biological_process	Wnt signaling pathway
A	0061630	molecular_function	ubiquitin protein ligase activity
A	0072572	molecular_function	poly-ADP-D-ribose binding
B	0000122	biological_process	negative regulation of transcription by RNA polymerase II
B	0000209	biological_process	protein polyubiquitination
B	0004842	molecular_function	ubiquitin-protein transferase activity
B	0005515	molecular_function	protein binding
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
B	0016567	biological_process	protein ubiquitination
B	0030514	biological_process	negative regulation of BMP signaling pathway
B	0031398	biological_process	positive regulation of protein ubiquitination
B	0032991	cellular_component	protein-containing complex
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0061630	molecular_function	ubiquitin protein ligase activity
B	0061631	molecular_function	ubiquitin conjugating enzyme activity
B	0070936	biological_process	protein K48-linked ubiquitination
B	1904262	biological_process	negative regulation of TORC1 signaling
C	0008270	molecular_function	zinc ion binding
C	0016055	biological_process	Wnt signaling pathway
C	0061630	molecular_function	ubiquitin protein ligase activity
C	0072572	molecular_function	poly-ADP-D-ribose binding
D	0000122	biological_process	negative regulation of transcription by RNA polymerase II
D	0000209	biological_process	protein polyubiquitination
D	0004842	molecular_function	ubiquitin-protein transferase activity
D	0005515	molecular_function	protein binding
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0016567	biological_process	protein ubiquitination
D	0030514	biological_process	negative regulation of BMP signaling pathway
D	0031398	biological_process	positive regulation of protein ubiquitination
D	0032991	cellular_component	protein-containing complex
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
D	0061630	molecular_function	ubiquitin protein ligase activity
D	0061631	molecular_function	ubiquitin conjugating enzyme activity
D	0070936	biological_process	protein K48-linked ubiquitination
D	1904262	biological_process	negative regulation of TORC1 signaling

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	BINDING SITE FOR RESIDUE V3L A 201

Chain	Residue
A	LYS61
A	ARG161
A	ARG163
A	LYS175
A	GLY179
A	HOH302
A	HOH305
A	HOH306
A	HOH318
A	HOH330
A	TYR107
A	GLY109
A	ARG110
A	TRP114
A	TYR116
A	ILE139
A	TYR144
A	GLN153

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 202

Chain	Residue
A	CYS36
A	CYS39
A	CYS56
A	CYS59

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 203

Chain	Residue
A	CYS51
A	HIS53
A	CYS70
A	CYS73

---

site_id	AC4
Number of Residues	19
Details	BINDING SITE FOR RESIDUE V3L C 201

Chain	Residue
C	LYS61
C	TYR107
C	GLY109
C	ARG110
C	TRP114
C	TYR116
C	ILE139
C	TYR144
C	GLN153
C	ARG161
C	ARG163
C	LYS175
C	GLY179
C	HOH301
C	HOH303
C	HOH304
C	HOH319
C	HOH320
C	HOH345

---

site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN C 202

Chain	Residue
C	CYS36
C	CYS39
C	CYS56
C	CYS59

---

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN C 203

Chain	Residue
C	CYS51
C	HIS53
C	CYS70
C	CYS73

---

Functional Information from PROSITE/UniProt

site_id	PS00183
Number of Residues	16
Details	UBC_1 Ubiquitin-conjugating (UBC) active site signature. YHPNInsn.GsICLdiL

Chain	Residue	Details
B	TYR74-LEU89

---

site_id	PS00518
Number of Residues	10
Details	ZF_RING_1 Zinc finger RING-type signature. CkHvFCylCV

Chain	Residue	Details
A	CYS51-VAL60

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	152
Details	Domain: {"description":"WWE","evidences":[{"source":"PROSITE-ProRule","id":"PRU00248","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	76
Details	Zinc finger: {"description":"RING-type","evidences":[{"source":"PROSITE-ProRule","id":"PRU00175","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	14
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	10
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"UniProtKB","id":"Q9NTX7","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	292
Details	Domain: {"description":"UBC core","evidences":[{"source":"PROSITE-ProRule","id":"PRU00388","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Active site: {"description":"Glycyl thioester intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU00388","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10133","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---