4QAF

Crystal structure of an engineered lipocalin (Anticalin) in complex with VEGF(8-109)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Lipocalin-1	polymer	152	16184.3	2	UniProt (P31025) Pfam (PF00061) In PDB	Homo sapiens (human)	Tear lipocalin, Tlc, Tear prealbumin, TP, Von Ebner gland protein, VEG protein
2	C, D	Vascular endothelial growth factor A	polymer	112	13149.0	2	UniProt (P15692) Pfam (PF00341) In PDB	Homo sapiens (human)	VEGF-A, Vascular permeability factor, VPF
3	A, B	10-{(1R,2R)-2-[(2E)-hex-2-en-1-yl]cyclopropyl}decanoic acid	non-polymer		294.5	2	Chemie (OMA)
4	C, D	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
5	D	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
6		water	water		18.0	210	Chemie (HOH)

Sequence modifications

A, B: 5 - 156 (UniProt: P31025)

PDB	External Database	Details
Val 26	Arg 44	engineered mutation
Gly 27	Glu 45	engineered mutation
Ala 28	Phe 46	engineered mutation
Leu 29	Pro 47	engineered mutation
Arg 30	Glu 48	engineered mutation
Cys 31	Met 49	engineered mutation
Leu 32	Asn 50	engineered mutation
Ala 33	Leu 51	engineered mutation
Gly 34	Glu 52	engineered mutation
Ile 37	Thr 55	engineered mutation
Thr 39	Met 57	engineered mutation
His 56	Leu 74	engineered mutation
Lys 58	Ser 76	engineered mutation
Ser 61	Cys 79	engineered mutation
Ser 69	Glu 87	engineered mutation
Ile 76	Lys 94	engineered mutation
Ile 80	Asp 98	engineered mutation
Ile 83	Lys 101	engineered mutation
Lys 87	Tyr 105	engineered mutation
Gly 89	Ile 107	engineered mutation
Ser 101	Cys 119	engineered mutation
Cys 104	Glu 122	engineered mutation
Ser 106	His 124	engineered mutation
Val 108	Lys 126	engineered mutation
Pro 111	Arg 129	engineered mutation
Trp 114	Lys 132	engineered mutation
Ser 153	Cys 171	engineered mutation

C, D: 8 - 109 (UniProt: P15692)

PDB	External Database	Details
Ser 110	-	expression tag
Ala 111	-	expression tag
Trp 112	-	expression tag
Ser 113	-	expression tag
His 114	-	expression tag
Pro 115	-	expression tag
Gln 116	-	expression tag
Phe 117	-	expression tag
Glu 118	-	expression tag
Lys 119	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	58666.6
Non-Polymers*	Number of molecules	6
	Total formula weight	936.2
All*	Total formula weight	59602.8

*Water molecules are not included.