4Q66

Structure of Exomer bound to Arf1.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, J, G, D	Chs5p	polymer	368	41010.4	4	UniProt (W7PD87) In PDB	Saccharomyces cerevisiae R008 (Baker's yeast)	Protein CAL3
2	K, E, B, H	Protein BCH1	polymer	739	83972.3	4	UniProt (Q05029) Pfam (PF09295) In PDB	Saccharomyces cerevisiae (Baker's yeast)	BUD7 and CHS6 homolog 1
3	F, L, C, I	ADP-ribosylation factor 1	polymer	175	19878.6	4	UniProt (P11076) Pfam (PF00025) In PDB	Saccharomyces cerevisiae (Baker's yeast)
4	F, L, C, I	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	non-polymer		522.2	4	Chemie (GNP)
5	F, L, C, I	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)

Sequence modifications

A, J, G, D: 2 - 364 (UniProt: W7PD87)

PDB	External Database	Details
Met -3	-	initiating methionine
Asp -2	-	expression tag
Pro -1	-	expression tag
Glu 0	-	expression tag
Phe 1	-	expression tag

K, E, B, H: 1 - 724 (UniProt: Q05029)

PDB	External Database	Details
Gly 725	-	expression tag
Thr 726	-	expression tag
Glu 727	-	expression tag
Asn 728	-	expression tag
Leu 729	-	expression tag
Tyr 730	-	expression tag
Phe 731	-	expression tag
Gln 732	-	expression tag
Gly 733	-	expression tag
His 734	-	expression tag
His 735	-	expression tag
His 736	-	expression tag
His 737	-	expression tag
His 738	-	expression tag
His 739	-	expression tag

F, L, C, I: 18 - 181 (UniProt: P11076)

PDB	External Database	Details
Met 7	-	expression tag
Thr 8	-	expression tag
Glu 9	-	expression tag
Asn 10	-	expression tag
Leu 11	-	expression tag
Tyr 12	-	expression tag
Phe 13	-	expression tag
Gln 14	-	expression tag
Gly 15	-	expression tag
Ser 16	-	expression tag
Gly 17	-	expression tag
Leu 71	Gln 71	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	579445.2
Non-Polymers*	Number of molecules	8
	Total formula weight	2186.0
All*	Total formula weight	581631.2

*Water molecules are not included.