4Q66
Structure of Exomer bound to Arf1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-02 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 114.659, 208.168, 155.078 |
| Unit cell angles | 90.00, 105.48, 90.00 |
Refinement procedure
| Resolution | 43.169 - 3.354 |
| R-factor | 0.2796 |
| Rwork | 0.279 |
| R-free | 0.31040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.501 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.410 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Number of reflections | 97079 | |
| <I/σ(I)> | 11.35 | 1.45 |
| Completeness [%] | 98.1 | 78 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | Exomer and Arf1 were mixed 2:1 in 200 mM NaCl, 50 mM Tris, 11% PEG 10000, 10% glycerol., pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
