4PQU

Crystal structure of HIV-1 Reverse Transcriptase in complex with RNA/DNA and dATP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	HIV-1 Reverse Transcriptase, p66 subunit	polymer	556	64022.4	2	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075)	Human immunodeficiency virus type 1 (HIV-1)	Reverse transcriptase/ribonuclease H, Exoribonuclease H, p66 RT
2	B, D (B, D)	HIV-1 Reverse Transcriptase, p51 subunit	polymer	428	50039.5	2	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815)	Human immunodeficiency virus type 1 (HIV-1)	p51 RT
3	E, G (T, E)	5'-R(*AP*UP*GP*GP*UP*CP*GP*GP*CP*GP*CP*CP*CP*GP*AP*AP*CP*AP*GP*GP*GP*AP*CP*UP*GP*UP*G)-3'	polymer	27	8759.3	2
4	F, H (P, F)	5'-D(*AP*CP*AP*GP*TP*CP*CP*CP*TP*GP*TP*TP*CP*GP*GP*GP*CP*GP*CP*CP*G)-3'	polymer	21	6416.1	2
5	I, S (A, C)	2'-DEOXYADENOSINE 5'-TRIPHOSPHATE	non-polymer		491.2	2	Chemie (DTP)
6	J, K, T, U (A, C)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
7	L (A)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
8	M, N, O, P, R... (A, B, C, D)	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
9	Q (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
10	AA, BA, CA, DA, EA... (C, D, T, P, E...)	water	water		18.0	435	Chemie (HOH)

Sequence modifications

A, C: 1 - 554 (UniProt: P03366)

PDB	External Database	Details
Met -1	-	expression tag
Val 0	-	expression tag
Cys 258	Gln 857	engineered mutation
Ser 280	Cys 879	engineered mutation
Asn 498	Asp 1097	engineered mutation

B, D: 1 - 428 (UniProt: P03366)

PDB	External Database	Details
Ser 280	Cys 879	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	258474.6
Non-Polymers*	Number of molecules	16
	Total formula weight	2002.3
All*	Total formula weight	260476.8

*Water molecules are not included.