4NQW

Structure of Mycobacterium tuberculosis extracytoplasmic function sigma factor SigK in complex with the cytosolic domain of its cognate anti-sigma factor RskA

Replaces:  3VDO

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	ECF RNA polymerase sigma factor SigK	polymer	204	22971.7	1	UniProt (O53730) Pfam (PF04542) Pfam (PF04545) UniProt (by SIFTS) (P9WGH7)	Mycobacterium tuberculosis	ECF sigma factor SigK, Alternative RNA polymerase sigma factor SigK, RNA polymerase sigma-K factor, Sigma-K factor
2	B (B)	Anti-sigma-K factor RskA	polymer	80	8555.5	1	UniProt (O53729) Pfam (PF22618) UniProt (by SIFTS) (P9WGX5)	Mycobacterium tuberculosis	Regulator of SigK, Sigma-K anti-sigma factor RskA
3	C, D, E, F, G... (A, B)	CADMIUM ION	non-polymer		112.4	10	Chemie (CD)
4	M, N (A, B)	water	water		18.0	89	Chemie (HOH)

Sequence modifications

A: 1 - 187 (UniProt: O53730)

PDB	External Database	Details
Met -16	-	expression tag
Gly -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Ser -6	-	expression tag
Gln -5	-	expression tag
Asp -4	-	expression tag
Pro -3	-	expression tag
Asn -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	31527.2
Non-Polymers*	Number of molecules	10
	Total formula weight	1124.1
All*	Total formula weight	32651.3

*Water molecules are not included.