4NQW

Structure of Mycobacterium tuberculosis extracytoplasmic function sigma factor SigK in complex with the cytosolic domain of its cognate anti-sigma factor RskA

Replaces:  3VDO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-01-28
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	57.390, 57.390, 150.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.690 - 2.400
R-factor	0.21018
Rwork	0.205
R-free	0.25986
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.842
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.695	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.093	0.339
Number of reflections	9747
<I/σ(I)>	20.1	8.1
Completeness [%]		100
Redundancy	14.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microbatch underoil	7.5	295	0.1M HEPES, 1M sodium acetate trihydrate, 0.05M cadmium sulphate hydrate, 12%(v/v) 2,5 hexanediol, pH 7.5, Microbatch underoil, temperature 295K