4NEE

crystal structure of AP-2 alpha/simga2 complex bound to HIV-1 Nef

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	D, L, F, I	AP-2 complex subunit sigma	polymer	142	17038.7	4	UniProt (P62744) Pfam (PF01217) In PDB	Rattus norvegicus (brown rat rat rats)	Adapter-related protein complex 2 subunit sigma, Adaptor protein complex AP-2 subunit sigma, Clathrin assembly protein 2 sigma small chain, Clathrin coat assembly protein AP17, Clathrin coat-associated protein AP17, Plasma membrane adaptor AP-2 17 kDa protein, Sigma-adaptin 3b, Sigma2-adaptin
2	G, B, J, A	AP-2 complex subunit alpha-2	polymer	398	44389.3	4	UniProt (Q66HM2) Pfam (PF01602) UniProt (by SIFTS) (P18484) In PDB	Rattus norvegicus (brown rat rat rats)	Adaptor-related protein complex 2, alpha 2 subunit
3	E, H, K, C	Protein Nef	polymer	155	17671.0	4	UniProt (Q90VU7) Pfam (PF00469) UniProt (by SIFTS) (P04601) In PDB	Human immunodeficiency virus 1

Sequence modifications

G, B, J, A: 1 - 396 (UniProt: Q66HM2)

PDB	External Database	Details
Gly -1	-	cloning artifact
Ala 0	-	cloning artifact

E, H, K, C: 63 - 203 (UniProt: Q90VU7)

PDB	External Database	Details
Gly 48	-	expression tag
Unk 49	-	expression tag
Unk 50	-	expression tag
Unk 51	-	expression tag
Unk 52	-	expression tag
Unk 53	-	expression tag
Unk 54	-	expression tag
Unk 55	-	expression tag
Unk 56	-	expression tag
Unk 57	-	expression tag
Unk 58	-	expression tag
Unk 59	-	expression tag
Unk 60	-	expression tag
Unk 61	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	316395.9
All*	Total formula weight	316395.9

*Water molecules are not included.