4N56
Binary complex structure of Klenow fragment of Taq DNA polymerase I707L mutant (Cs3C KlenTaq) with DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | 5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(DOC))-3' | polymer | 12 | 3617.4 | 1 | |||
| 2 | B (C) | 5'-D(*AP*AP*AP*GP*GP*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3' | polymer | 16 | 4964.2 | 1 | |||
| 3 | C (A) | DNA polymerase I, thermostable | polymer | 553 | 62434.8 | 1 | UniProt (P19821) | Thermus aquaticus | Taq polymerase 1 |
| 4 | D (C) | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) | |||
| 5 | E (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | F (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 7 | G, H, I (B, C, A) | water | water | 18.0 | 21 | Chemie (HOH) |
Sequence modifications
A: 281 - 832 (UniProt: P19821)
| PDB | External Database | Details |
|---|---|---|
| Met 280 | - | initiating methionine |
| Leu 707 | Ile 707 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 71016.4 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 171.3 | |
| All* | Total formula weight | 71187.7 |
